Effect of actinophages on growth of actinomycete isolate and production of bioactive metabolites.

bolites was investigated in the present study. Moreover, to protect actinomycete against actinophage
attack immobilization in alginate beads was used. Presence of actinophages in the liquid culture of
actinomcete (AC2) free cells, markedly reduced the fresh and dry weight of its mycelium as compared to
the free cells culture in absence of actinophages. Whereas, the highest fresh and dry weight of mycelium
were recorded in alginate immobilized culture. In case of actinomycete immobilized cells, presence of
actinophages had no marked effect on the fresh and dry weight of mycelium. The highest amount of
metabolites were produced by immobilized actinomycete as compared to that produced by the free cells.
Presence of actinophages had no marked effect on the amount of metabolites produced by immobilized
cells. Using gas chromatography–mass spectrometry (GC-MS) analysis, five chemical compounds were
detected in metabolites of actinomycete isolate (AC2).