Actinophages of an actinomycete isolate of high antagonistic activity against certain pathogenic bacteria. (Isolation and characterization).

Abstract

Twelve isolates of actinomycetes were isolated from soil and compost samples collected from Minia Governorate Egypt and designated AC1, AC2,…upto AC12. Actinomycete isolate AC2 exhibited high antagonistic activity against Staphylococcus aureus, Escherichia coli, Klebselia sp. and Bacillus cereus. actinophages specific to actinomycete AC2 were isolated from soil samples collected from Minia Governorate. Twelve single plaques ofactinomyceteviruses (actinophages), were isolated. Due to the similarity of the actinophage isolates in their optimum pH sensitivity to UV (260 nm), thermal inactivation point and host range theseactinomyceteviruses (phages) were classified into groups (group A and group B). On the basis of electron microscope study, micrographs of actinophages of every group were found to be similar in their size and morphology. Generally, the obtained results indicated that, the twelve isolates of actinophage were found to be belonging to two types of phages. These two phage types were named ØAC2a and ØAC2b.

Keywords

Minia Journal of Agricultural Research and Development
Volume 45, Issue 4
December 2025
Pages 517-530

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