Characteristics of Bacteriophages specific to salt tolerant Azotobacter sp. in Minia Soils.

Abstract

Five isolates of Azotobacter were isolated from different locations in Minia Governorate - Egypt and
designated Az1, Az2 Az3, Az4 and Az5. Az3 was found to be most tolerant isolate to NaCl. Bacterial
viruses of Azotobacter (Az3) were isolated from soil samples collected from Minia Governorate. Twelve
single plaques of Azotobacter phages, were isolated. Due to the similarity of the phage isolates in their
optimum pH sensitivity to UV (260 nm), thermal inactivation point and host range the phages of
Azotobacter were classified into groups A and B. On the basis of electron microscope study, Azotobacter
phages of every group were found to be similar in their size and morphology. Generally, the obtained
results indicated that, the twelve isolates of Azotobacter phage belong to two types of phage. These two
phage types were named Az 1, Az 2.

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