METABOLITES PROFILING AND IN VITRO ANTICANCER AND ANTIOXIDANT ACTIVITIES OF THREE DIFFERENT MARINE SPONGES METHANOLIC EXTRACTS

In the current study, three different marine sponges methanolic extracts
Clathria arbuscula (SR), Crella cyathophora (S), and Xenia macrspiculata
(CS) were collected from the Red Sea in Hurghada and examined for their
metabolite profiles, antioxidant, and anticancer properties. Flavonoid, total
phenolic , (2,2-diphenyl-1-picrylhydrazyl )DPPH, and ( Ferric Reducing
Antioxidant Power)FRAP tests for antioxidants were used to identify the
different types of marine sponges methanolic extracts. The anticancer action
of the cancer cells MDA-MB 231, HUH-7, and NB4-APL was dose- and
extract-dependent. Total phenolic content (TPC) of Xenia macrspiculata
methanolic extract has been found to be higher than that of Clathria
arbuscula methanolic extract and Crella cyathophora methanolic extract .
While the total flavonoid content (TFC) of Xenia macrspiculata methanolic
extract has been shown to be higher than that of Clathria arbuscula
methanolic extract and Crella cyathophora methanolic extract. Different
levels of FRAP and DPPH activity have been found in all methanolic
extracts of marine sponges. It has been worked the UPLC-TOF-MS analysis
for the three different extracts. A total of 1,054 metabolites were found in the
metabolome analysis. It was divided the primary groups of chemicals were
and peptides, amino acids ,fatty acids and conjugates, organic dicarboxylic
acids, purines, benzoic acids, indoles, pyrimidines, benzenes,
phenylacetic acids, and pyridines compounds. The multivariate statistical
analysis identified 32 metabolites, including 2-Chloro-N,
N-dimethyl- 3- oxobutanamide, 4-Methyl-2-oxo-pentanoic acid, ,6-
Dihydrouracil, 3-Hydroxy-3-methylglutaric acid, (E)-3-hydroxy-2-
(2-hydroxypentadecanoylamino) dec-4-ene-1-sulfonic acid, Cysteine
sulfinic acid,10-Hydroxydecanoic acid, 1-Aminocyclopropane-1-