ASSESSING THE IN VITRO ANTIOXIDANT AND CYTOTOXIC PROPERTIES OF ALPINIA OFFICINARUM AND HUMULUS LUPULUS EXTRACTS

BSTRACT
In this study, we investigated the cytotoxic and antioxidant properties
of acetone extracts obtained from Alpinia officinarum (Al. officinarum) and
Humulus lupulus (H. lupulus). The assessment encompassed the
computation of the extracts' total flavonoid content (TFC) and total phenolic
content (TPC), revealing noteworthy concentrations of both phenolic and
flavonoid components. According to DPPH and FRAP tests, both extracts
had high antioxidant activity. Their low IC50 values suggested that they were
capable of scavenging free radicals. Several bioactive substances were
detected in each extract by gas chromatography-mass spectrometry (GCMS) analysis. These chemicals included ç-Sitosterol and n-Hexadecanoic
acid in Al. officinarum and vitamin E and α-amyrin in H. lupulus. These
results point to the plant extracts' potential for therapeutic use. Furthermore,
assessments of the cytotoxicity of hepatocellular carcinoma (HepG2), lung
cancer (A549), and breast cancer cell lines (MCF-7). The Al. officinarum
extract shown strong cytotoxicity against each of the three cell lines, with
IC50 values for each cell line ranging from 107.10 μg/mL to 51.57 μg/mL
and greater than 100 μg/mL. H. lupulus extract exhibited cytotoxic
properties as well, with IC50 values greater than 100 μg/mL. These results
highlight the potential of extracts from Al. officinarum and H. lupulus as
sources of bioactive components with cytotoxic and antioxidant qualities,
indicating their potential use in the creation of innovative medications. The
significance of medicinal plants in the search for novel therapeutic
molecules is highlighted by this study.