EFFECT OF PLANT GROWTH REGULATORS ON ORGANOGENESIS OF SALVIA OFFICINALIS L. PLANTS

The traditional applications of sage herb in the domestic medicine are endless moreover, it is widely known as an ornamental plant. Nodal and leaf explants were cultured on MS medium supplemented with different combinations of TDZ, BA or Kin as a source of cytokinin and IAA or 2,4-D as an auxin. Supplemented growth medium with TDZ promoted callugensis of both types of explants however, callus did not develop when the medium contained only BA or Kin. Number of developing shoots was significantly varied among concentration of PGRs. Nodal explants cultured on medium with 1.5 mg/l TDZ + 0.1 or 0.5 mg/l IAA had the highest significant shoot number (7.2 shoots/explant) followed by IAA + TDZ at 1 mg/l which developed 5.5 and 5.8 shoots/explant, respectively. In many cases the adventitious shoots failed to develop into shoots even after subculture. The tallest shoots 9.6 cm were observed on medium with 1 mg/l of BA or Kin + 0.5 mg/l IAA. Adventitious shoots were perceived for all leaf explants that cultured on medium supplemented with (TDZ + IAA) and (1 mg/l TDZ + 0.1 mg/l 2,4-D). Unlike TDZ, Kin and BA had low efficiency to sustain shoot growth as the number of developing shoots was 0.4/explant. This method of regeneration will facilitate selection of S. officinalis plants more tolerant to environmental stress, their micropropagation, and the in vitro production of secondary products.
 
Abbreviations:2,4-D-dichlorophenoxyacetic acid; BA – 6-benzyladenine; IAA – indole 3-acetic acid; Kin -Kineten; MS –Murashige and SkoogTDZ – Thidiazuron;